PCR-RFLP of IGFBP-3 Gene in Some Egyptian Sheep Breeds

The present investigation was to study DNA polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene by PCR-RFLP in four Egyptian local sheep breeds. Genomic DNA was isolated from a total of 20 animals from four Egyptian breeds (five each) of sheep namely Rahmani, Ossimi, Awassi, and Barki. A fragment of IGFBP-3 gene, comprising of a part of exon 2, complete intron 2, exon 3, and a part of intron 3, was amplified. The amplified fragment was found to be 654 bp in sheep. On digestion of 654 bp with Hae III restriction enzyme yielded single restriction pattern of five fragments of sizes 201, 201, 87, 67, 57 bp in all the animals belonging to the four Egyptian breeds studied revealing absence of polymorphism in those four Egyptian sheep breeds.